- Alonso Ravens (Tampa)
- Apopka Blue Darters
- Belleview Rattlers
- Choctawhatchee Indians (Fort Walton Beach)
- Crestview Bulldogs
- Dr. Phillips Panthers (Orlando)
- Durant Cougars (Plant City)
- FAMU DRS Baby Rattlers (Tallahassee)
- Florida High Seminoles (Tallahassee)
- Gadsden County Jaguars (Havana)
- Godby Cougars (Tallahassee)
- Jefferson County Tigers (Monticello)
- Lake Placid Green Dragons
- Leon Lions (Tallahassee)
- Lincoln Trojans (Tallahassee)
- Madison County Cowgirls (Madison)
- Miami Jackson Generals
- Munroe Day Bobcats (Quincy)
- Niceville Eagles
- Rickards Raiders (Tallahassee)
- Robinson Knights (Tampa)
- St. Cloud Bulldogs
- Tarpon Springs Spongers
- Wakulla War Eagles (Crawfordville)
- Wharton Wildcats (Tampa)